Nice people thread part 4 - sugar and spice and all things

vivatifosi

I used to do non-fiction selection at my old library and loved it. We'd take it very seriously and tried to get the right blend of items that reflect customer demand (what's popular in one library isn't necessarily popular in another); seasonal trends; current interest (eg books about maths at the moment are popular due to the Du Sautoy programmes), and generally off the wall "different" things that you think people might like.

Nikkster

misskool wrote: »

ask him to explain using jimmy choo analogies and see how well he does

It's simple molecular biology, except i'm doing it with something slightly more complicated and shouldn't matter, except it's JUST NOT WORKING.

At the risk of being seen as some kind of nice people stalker (I promise I'm not, I'm just to shy to contribute much), I work with people who do a lot of pcr... is it real-time? what are your problems? I've only tried it a couple of times (with a lot of assistance), but my colleages are literally doing pcr just about every day. Feel free to pm me with more details if you want (or not if you don't, I won't be offended).

Davesnave

lostinrates wrote: »

aww.

I thought you were writing. Davesnave has a good cautionary tale for a smallholding mag that I think would make a good piece for you I reckon.

Yes, I'd caution against ever getting into smallholding!

misskool

Nikkster wrote: »

At the risk of being seen as some kind of nice people stalker (I promise I'm not, I'm just to shy to contribute much), I work with people who do a lot of pcr... is it real-time? what are your problems? I've only tried it a couple of times (with a lot of assistance), but my colleages are literally doing pcr just about every day. Feel free to pm me with more details if you want (or not if you don't, I won't be offended).

Aw..thanks.

Real-time is easy.
I'm trying to amplify bisulfite-treated DNA to determine methylation status and I can get one set of primers to work but not the other set. There is a trick I'm missing but I'm not sure what. If any of that makes sense, I can PM with more details.

Nikkster

misskool wrote: »

Aw..thanks.

Real-time is easy.
I'm trying to amplify bisulfite-treated DNA to determine methylation status and I can get one set of primers to work but not the other set. There is a trick I'm missing but I'm not sure what. If any of that makes sense, I can PM with more details.

They don't do so much real-time which was why I asked
I think that they do something (or are planning to do so) regarding methylation status. I might have mis-remembered though.

Feel free to pm me, chances are that they won't be able to help with your problems but you never know!

Nikkster

Just to explain the comment about being a nice-people stalker... I've:
1. 'rolled an easter egg'
2. worked on the census
3. seen fc's pop-up shop
4. was offered some daily mail numbers from last weekend
and I
5. work in science
I think thats about it

I've lurked on the house prices board since before it became 'hidden'

fc123

Hi Nikkster :hello: Don't be shy......If you contribute more then I can add to my repertoire of things I have learnt on this thread that I have zero real life experience of........I may learn more about PCR's to go with small holding info, feral chickens, teens with learning difficulties, how librarians select books and help their customers, how to move to 2 (or was it 3?) houses within 4 weeks, F1, sheep, caring for ill parents.....and all the other things that go on here.

Makes me feel more of a rounded human being rather than being trapped in my little bubble world sometimes.

Doozergirl wrote: »

The unpacking is indeed nearly done and I am now trying to get it ready to move again. There was one house for sale, we moved to it to sell it, back home again because it was too far, put home up for sale as well, sold it first so we have moved again to the house we were originally trying to sell! That is going through and the money from both will buy the house where we hope to build a house. Not confusing at all!

The new house is a lot smaller than the combined 4100 square feet that we've had of late (not including garages and cellarage) so I am now onto trying to consolidate before we get there! :eek: I thought we lived fairly minimally, but we don't. I clearly have a thing for furniture. I have more furniture than stuff to put in it.

Does anyone want a Habitat Stella dining table going cheap?! I have two

I think I get it now....sort of.:)

PasturesNew wrote: »

No, hammered silver. It's lovely And small ... and simple. Like me: small and simple.

I did a practice piece in bronze first - and will be making some bronze ones when I get my own hammer at home.

Sounds like a great way to spend a week.......and brave of you too to spend a week with strangers. I would struggle with a week like that myself and am not speshul at all. Did you eat some fruit or fresh veg today?


Liking the holiday tales too SS and MS....was thinking about you both today as the rain chucked it down in London for hours on end.

We have the trade show this weekend and OMG I am wired to to the max........the entire range just didn't climb out of my head in time (by that I mean a fully factory ready pattern and sealed sample ready to go) but some did.
Dreading being 'on display' for 3 days to all the trade...it feels like being naked in public.

PasturesNew

misskool wrote: »

I'm trying to amplify bisulfite-treated DNA to determine methylation status and I can get one set of primers to work but not the other set. There is a trick I'm missing but I'm not sure what. If any of that makes sense, I can PM with more details.

Ah, yes, that's a fundamental error there - common among newbies.... I'll PM you the answer of course... in a minute.

michaels

It amazes me how quickly you turn this stuff round FC - I would imagine it would take weeks to put one garment together. I? am not a naturist but despite my pot belly I have no hang ups about being seen naked whereas for DW it is a huge deal, strange how we all have different things that worry us.

We had the rain last night, today was sun and cloud with a cool breeze - poor kids were freezing during their swimming lesson, the Med has a lot to be said for it. Tomorrow we are going to a theme park near Totnes - being MSE I have found a money off voucher. I am feeling uncomfortable about leaving the builders so long and am really starting to feel uncomfortable at my parents house - just the being in someone else's house thing so am quite ready to go home next Tuesday (if I can stick it out till then).

Citibank are I think FSA compensated and do a EUR account.

GDB2222 wrote: »

Are we in for another Global Financial Crash or is this just a correction? FTSE is down 3% today, and 7 - 8% on the week. Various talk that Italy will default on (ahem, I mean reschedule) its debt. Any thoughts kind people?

A very interesting question but don't we discuss this sort of stuff elsewhere on the board?

PasturesNew

fc123 wrote: »

Sounds like a great way to spend a week.......and brave of you too to spend a week with strangers. I would struggle with a week like that myself and am not speshul at all. Did you eat some fruit or fresh veg today?

They're all lovely - and we're all of the same mind re the tutor being .... chaotic/annoying/disorganised... and they have even been timing how long it takes to extract some metal out of him (most are experiencing 1-1.5 hour delays between asking for 6" of any metal and actually getting it in their hand

Today's diet was:
Sausages, eggs, beans, toast, coffee, jam (that's fruit, right!)
2 pastries (you get those with morning/afternoon coffee)
3 chocolate giant cookies (purchased Tuesday night)
Pie/chips.

it feels like being naked in public.

Luckily I don't know how that feels.....